Best Practice & Research Clinical Haematology
Volume 22, Issue 3 , Pages 355-365, September 2009

Standardisation of molecular monitoring for chronic myeloid leukaemia

  • Nicholas C.P. Cross, MA, PhD, FRCPath (Professor)

      Affiliations

    • National Genetics Reference Laboratory (Wessex), Salisbury, UK
    • Human Genetics Division, School of Medicine, University of Southampton, UK
    • Corresponding Author InformationNational Genetics Reference Laboratory (Wessex), Salisbury NHS Foundation Trust, Salisbury SP2 8BJ, UK. Tel.: +44 1722 429080; Fax: +44 1722 338095.

Molecular monitoring of chronic myeloid leukaemia (CML) patients by real time quantitative reverse transcriptase PCR (RQ-PCR) is of clinical value, but the use of diverse laboratory protocols and units of measurement make it difficult to compare results between and sometimes within centres. This review explores the intrinsic difficulties in standardising the RQ-PCR analysis, summarises the progress that has been made following the proposal for a new International Scale for BCR-ABL measurement and discusses how further improvements are likely to be made.

Keywords: BCR-ABL, real time PCR, international scale, minimal residual disease

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PII: S1521-6926(09)00020-6

doi:10.1016/j.beha.2009.04.001

Best Practice & Research Clinical Haematology
Volume 22, Issue 3 , Pages 355-365, September 2009

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